基因数据处理50之cs-bwamem、bwa、snap、bwa-mem与art比较

(5):bwa-mem：

hadoop@Master:~/cloud/adam/xubo/data/GRCH38Sub/cs-bwamem$ cat G38L100c50Nhs20.bwamem.sam 
@SQ SN:chr1 LN:248956422
@PG ID:bwa  PN:bwa  VN:0.7.13-r1126 CL:bwa mem GRCH38BWAindex/GRCH38chr1L3556522.fasta fastq/g38L100c50Nhs20.fastq
chr1-50 0   chr1    93465785    26  100M    *   0   0   TTCCACAATAGTTGAACTAATTTACAGTCCCACCAACAGTGTAAAAGTGTTCCTATTTCTCCACATCCTCTCCAGCACCTGTTGTTTCCTGACTTTTTAA    @@CDFDFDHFHGHIJH:IJJJ(JJE?JDIDEJIB@FGJIGBHJ()HG8(CIICGFFHEH=GI3@&@DD58FADDACHDDHFCD8D,DCC<CEFD<EDDCD    NM:i:0  MD:Z:100    AS:i:100    XS:i:90
chr1-48 0   chr1    228133746   60  100M    *   0   0   ATCATTGTATGCCACAGAAATAATTAAATTTCCTTGTCAACTGACACATTATTATTAGGCACTCTCACCAGATCTTTACCCATGGCCATTTAAAGTGTGG    @>CFFFFFH<<GC1IIDCFJJHIGIHJ(IID7IJ,FJJJHJJJJ)GGBHIJFJFIFIHFE=HEIEE;CA)G0(D()HC@D(:EFDDC@;DDAC95(D?BD    NM:i:1  MD:Z:44G55  AS:i:95 XS:i:19
chr1-47 0   chr1    13772988    60  100M    *   0   0   TTCAGTAATTCAGAATAACACATGAGGGAATGAATGAATGAATAAATAAAAAAAAACTGAATGAATAAATTACAAAAAATTGTGTTTCAGGGAAGAAAAA    CC@F(FFFDFH.HDHIGI(JIIIGGIEEJIIIHJJHHH3IJJIIJ3=EI>JDIGH((IBJCIEHGD>;J@HF+DC)CCCADBDBD+BDDDD5B5DDDE(C    NM:i:2  MD:Z:56A15A27   AS:i:90 XS:i:23
chr1-46 16  chr1    37474758    60  100M    *   0   0   GGGTCGGGGTCCTGTTCCCCGGTCCGCCGGGCCTCAGGACCCCTCCAACTTTGCCCAAGTTGGGAGAGCCGGGGAAGAGCACCAGGTTCCTGATCGGGAT    (5CBACDDD>FBDDDDDEC:CE(CBDFDDHEFH;FGEFHGHDGJJJDIGI:JEHJ=JJJJJH8CI?JJJG9JIII>IJIIGJ=EIJGAHHHHFFDFDCC?    NM:i:1  MD:Z:22C77  AS:i:95 XS:i:0
chr1-45 0   chr1    29056657    60  100M    *   0   0   CTGGGATTACAGGTGCCCGCCACCATGCCCAGCTAATTTTTGTATTTTTGGTAGAGACAAGGTTTCACCATGTTGGCCGGGATTGTCTCGAACTCCTGAT    B@@FFFFFHHG)HIJJJJBJIJCJHGJIBFJJI3IIHDF@JIAJ9JJJIJJBIJJ?BJID8F:HFHA(+D>J>CG>7D=DDFF@EDC3D<CDDDC@BD@B    NM:i:0  MD:Z:100    AS:i:100    XS:i:64
chr1-44 0   chr1    49993893    60  100M    *   0   0   CAATTTAGCCAAAACTGGCTAATCGTTTTACCAGAATCATTCCCATTGTTCAAGACCTATTTTAAGCTCCACTATCACCATAAAACTTTCCCGATCAGTT    C@CFFFFDHHHHH<IEJJ@JJJHI)IDIBIJA:HJHFJJJIJGGJJIIIIHGGJJGH<(IIIJI?ICDG;CDHFHCDCCB?FDDED:CD:>DD5C&DDCD    NM:i:1  MD:Z:24C75  AS:i:95 XS:i:0
chr1-43 16  chr1    194714506   60  100M    *   0   0   AATATGTTTTAATAATATCATATTTAAATTTGATGATACTTTAAAAATGGTTCCATGTGTGTTCTCTTGGGTTATTTCACAATCAATAAAAGGTCTGCAA    CCCCDDC@E>CDCDDC>D9CD=C)CGC>E@7.HF)DIBJBJJ.JEJEJ@JJIIIIGD?<IHH)FJJIIH*DJIBIIJHJIHHJFHIGHHHHDFDFDFC@B    NM:i:0  MD:Z:100    AS:i:100    XS:i:0
chr1-42 0   chr1    35706203    41  100M    *   0   0   CAGGTTCAAGCGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACGTGCCACCATGCCTGGCAATTTTTGTATTTTTAGTACAGATGGGG    CC@FDFFAHHGFHJIHJFJJII=@JEHIJIIJIJEJIJJHHGIJBBFJG6JJHJJG<F3JJHIFG(DCJDHDFDCHDDF7DDHBDFDDCDDCDCD;CBCB    NM:i:0  MD:Z:100    AS:i:100    XS:i:84 XA:Z:chr1,-93072856,28M1D72M,3;
chr1-41 16  chr1    156482338   60  100M    *   0   0   GTGTGTGCATAGGCAGGTCTGCGTGTACATGCAACGTGGGCACGTGTCCATGTGGATGCAGGCGGGGGTATATCCTGGTGCCTGTGTGTATGGGCCCACC    D;CCDDCDCDDDCD:EDA@<C<E(GDDDGDJDDHJ@CJJI,=FHJJIGJ7GEC?IGJJIFBBICHJEIJJHHAIJIJI.IJGJJGJJHHGHHFFFFFB=B    NM:i:0  MD:Z:100    AS:i:100    XS:i:0
chr1-40 16  chr1    221779284   60  100M    *   0   0   CATGGCACATAGCACTTTGGTGATGGGGACTGCTTTGCTAATGTCAGGGTCAAGGGGTGCATGGACCATGGGCAGAGTGCTGGGCTCAGCCAAATGGTTC    DDBCDCDDDDDDD25F?DD@4I5HED?CAHGA?JJIIJB)IHFJJFCJII?@<HIIFGIJIJFG?JIJCIIJJ)IJJIJIJJIIEGJFHHHFFDFDFC@@    NM:i:1  MD:Z:39G60  AS:i:95 XS:i:0
chr1-39 16  chr1    3895605 60  100M    *   0   0   GTCCTCTCCGGATTGACAGGAGTCAAAACATGAGATCGGCTTAGCTTCAGTTTCGTCATGGATTAACCACCTCCAAGGTGTCAACTCCAAAATGTCAAGA    DD5CCAD&8DAD>D&FDDDCDBDD?6DD.FHDDIFE?@IDEGIBCGD?JFJ>JGBI,IJIF.JJIHJJJEIEGFJ=JJHJHHJFHIJHHHHHFFAFFC@B    NM:i:0  MD:Z:100    AS:i:100    XS:i:0
chr1-38 16  chr1    33174926    60  100M    *   0   0   CACACATACATATATGTGTGTATATATATATATATATATATATACACACATATACATATATATGCACACACACATGTATGTATATGTATATGTATATGTG    CDDC(FBDC(AACBDDCBDDECEC5@H;HFDJFH>=FCHAHJFJ'H3JG9JFEHIJFDJJ9IJHEJIGJIJJJJJC;J?AJFJGEHFHDC<HDFFFDCC# NM:i:1 MD:Z:99A0 AS:i:99 XS:i:44 chr1-37 16 chr1 206124777 60 100M * 0 0 TCAGTCAATAGACATTTGGGTTGTTTCCACCTTGGGCAGGTTACAAATAATGCTGCTAGTGAACATTCATGTGCAAGTTTTTGTGTGGACATACGTTGTT CBCD8DDADC@DDDDB?CCHD;@AHECEEJIHAEII?E05GFJHHDJCJEJDBHJE7GJJJGJGGGJ=JIC(JIJHHIIIAGGJIIICHGHGFFFDF?CC NM:i:0 MD:Z:100 AS:i:100 XS:i:27 chr1-36 0 chr1 181673626 60 100M * 0 0 TCCACTGCCCAGAAAGAGGACATCCCTTATAGGACCAGCGGATGGAAGCCATGGGCTGGGCAGGACATTCCTGTCCCAACCCACATGGCAGCTAGAGTCC @@3DFFFFHHHFHJJIGGJHHFJJJIIJJJJDH*GJJJGJIJ6AIJIJDFDII=HFI2AH1AIEAAC?JEIEDJF.HDH@FAFDCDE2D:DDBD0DDBDD NM:i:1 MD:Z:33G66 AS:i:95 XS:i:0 chr1-35 16 chr1 152104780 60 100M * 0 0 ACCTCTATAAATACAATATCTTCAAATATGATTACATTCTGAGGTACTGAGGGTTAAGACTTCAACACGTGAACCTCTGTGGGGGTTGGGAGGTCACAAC +@?D>>D?DDBDFB)DDDDDC5(9>F;G)FB84/AJE3JJIJIGIGJBBIGCJCJGJGHJIDJ>IB7IGJGEGCIIGFJJJEFHIIJHHF=HFF8=F??= NM:i:1 MD:Z:14C85 AS:i:95 XS:i:32 chr1-34 16 chr1 12934213 0 100M * 0 0 TTTTGATACTTTTGATGTGGCCAAAGGTTCTCCAATAAAGATACCATATATAAATATATGTATTTCTAATGTCTGAAACAGATTAAAACCTTCCCTGTAT D@CB?DEDCEDDD(DC>F>DEHE>HEDE@HDD.IDD3'5I8IBFJHDI=CIIJ8JFHIBJJI0IJGFFJGIIJJABH<)IFJJJIEDHHHHAFFFFFCC@    NM:i:0  MD:Z:100    AS:i:100    XS:i:100    XA:Z:chr1,0;
chr1-33 0   chr1    48968233    60  100M    *   0   0   TAATAGTAGGCAATAAACAAAGAGAGCAACTTAGGAGCCAGATCACATGTGGCCGCTCGAGCAATATGGTAAAAGTTCTGGACTTCATTCTAGGTGAATG    1CCB=FFFHHHHHEDHJIIAFG4JIFJIJB)JJI?(&JJIJJEE)HIJJBJ?HJ(=B(I@?I?8DC8C>JHJH>@EDFDD5DDDDDDDDCFD:=DCC(DD    NM:i:2  MD:Z:0G53T45    AS:i:94 XS:i:0
chr1-32 0   chr1    88980623    60  100M    *   0   0   TAGTTCAGTAAACTATTTATCAAACAGGTGTCAGGTCATTTTAACATACTCCTTGCTTTGAACAATATTCATTCATACTTGGTACAAACTCTATATCCTA    B?CFDFFFHHFH3JIJJJIGJJJJJFJDEJGJ(EHFI>E=JIJ(GGJDFCH>>GJ=IHDJEHHDI>GEBJE@DD@HH'AA@ECC@BDEDDD@CDDADBDD NM:i:2 MD:Z:32T44T22 AS:i:90 XS:i:0 chr1-31 16 chr1 227005594 60 100M * 0 0 TCACCAGGCATCTTTACTGACTCACACCAATAGTAGTACTGGGATTAGAAATAAGACGCTGCAATACTCACAACCTAGGTGAAGTTAGTTAATTTGGGAA D@D5B=DACDDDDDBEFECBFDC5BCDDDCDFIDC8ICEIJ=DHIGHIJIJJB0HJJCDJHJGJIJI9GGHGGJ3@IJJAIJGGBGJ7HFHHDEBFF@CC NM:i:0 MD:Z:100 AS:i:100 XS:i:0 chr1-30 0 chr1 9852129 60 100M * 0 0 TGTGAAATGGAGTCAGCAGAGTGAGCCGGCCTCCACTCAGTGAGCCGGGTCTCCCCCACAGCCGGCATGTGCTGACCTCCTTCCAACTGCTCTACCAAGA CBCDDFFDHGHHHIEJ+J<EFJIJIIJI><J(IIFJG)0JGIJ?8J5;J?D@9IJHDI=DI)DDHG@3FAI5FF?EDAHDC@DDGD3AA>D+?ECDDDDB NM:i:0 MD:Z:100 AS:i:100 XS:i:0 chr1-29 16 chr1 156397431 60 100M * 0 0 TCAGCCTCCCGAGTAGCTGGGATTACAGGAACCTGCCACCACGCCCGGCTAATTTTTGTATTTTCAGTTGAGACGGGGTTTCACCATGTTGCCCAGGCTG D1D(@9DDDC@D0C3=CDDJ;FDHDD@H2BDHIDAGDDDCDIFJ9GIFGIG@?)JJHJGFGJIB7JG>'IJIJJGJ+JJGIIHFIJIDHHHFFFFFFC@B    NM:i:1  MD:Z:68A31  AS:i:95 XS:i:55
chr1-28 16  chr1    56986638    51  100M    *   0   0   ACTCAGAACAGGTCTCCTTGTGGAACCATGGCCTTCCTTTTGGATCCTGGCCATGAGAGCCCATTCTTAGGAACCATGTTTCAATTCCAGTAGGTGATGT    DD)DC@C<EDDD+DC0BDDBDDCECFDIJJ@)?HDACJGDFI?JGJH)JJJJJIEIJIIJJGIJIHIJHJCIGDHI>J@A)GIFJJJHHFFHFDDFFCCC    NM:i:0  MD:Z:100    AS:i:100    XS:i:80
chr1-27 16  chr1    172015198   60  100M    *   0   0   AGGTGTCAGTCCTCCAGCTTTGTTCTTCTTTTATATTGTGTTGGCTATCCTGGGCTCTTTGCTTCTCCATACAAAACTTAGAATCAGTTTGTTGATATCC    B8BD>/D<BED@CCEBBEBCH,F?CCD.E;HGJBJ)IGD7HED5@6JJJCHIGHJIJFDJCIJJHGJIJJJIEF:FEJHBJ.JJJIGHBHCF2DDFFCC@    NM:i:0  MD:Z:100    AS:i:100    XS:i:23
chr1-26 0   chr1    233336763   60  100M    *   0   0   AGATATACAGCAAAGTTTGAAAGCTACAGTTCTGAGGACCATATTTATGGATTCCTTCTTATATGTTATCTGGGTTGATATAGAAATTCTTCCATGGCTA    CBCFDFF<H<G?AIBJJJGIEJIJIIJIIJJEGHEIJIIGI)GJHIJGF8JIIHED=DJH?IJFB;>;HHDDHHB?C9DE?DCE@D?B&5E>DDD7DD?D    NM:i:1  MD:Z:41G58  AS:i:95 XS:i:0
chr1-25 16  chr1    105822718   0   100M    *   0   0   CAGCCAAAAAACACATGAAAAAATGCTCACCATCACTGGCCATCAGAGAAATGCAAATCAAAACCACAATGAGATACCATCTCACACCAGTTAGAATGGC    DDD>=D6FCDC?DDCDECHAEDD?DJIDE(EFE;:?DDG?JHJ>J3IIIHJHIHDEGJJJHHIGJIICH)GJIJIGIAJ:JFGICHHHHHGBFFFFDCCC    NM:i:0  MD:Z:100    AS:i:100    XS:i:100
chr1-24 0   chr1    235841969   60  100M    *   0   0   GTTGGCTACTAGCTTAGCAGAGGTGCAAAACCATGAATTTCTGGTGGTATGGATTTTTTCAGCTATTTCAGATTCACCAGCAGGATCCAGCTGCTTGGGT    CCCFF?FFFHHHFGI,CBD@DEC;?DDHD<BEED&DD@DCDEDDDAD?    NM:i:2  MD:Z:25G60T13   AS:i:90 XS:i:0
chr1-23 0   chr1    96545358    60  100M    *   0   0   AGTGAAAAAGGCTGGCTGCCCTTCAATATCATCTTCAAATGTTAACAACACTGAATATTAATAAATTTCCTTTAGCGAATAATGAATCCAGCCTTCCTTA    C@CF+FFFGGDHGJIBDJI2JGJIHHJJII?GJJJJGIJIJJGFJJG)IJ0HD0JIFJDJDFC;D7JGFFCEDFHADCDCCDEDDEAHDDD+9?<CA2:D    NM:i:0  MD:Z:100    AS:i:100    XS:i:0
chr1-22 0   chr1    80270679    60  100M    *   0   0   TTGTACACCCTATTTCTGACCAGAAGAAGGAGCATTTTGCTTTTTGCCAAATGAGAAGTGCATTCTGGAAACACTTGATGCCTGCACCACACCTCGAGTT    ?@CFDDFFHFHHHJJJGC7J(GI8IJJJE?HHI>BJG*IJFJIDJHD0IEJIHDI>@H=EHGIAHJ33(EJCDEDA?FDG<ADDDCDDEF9DDDD@DDBD    NM:i:0  MD:Z:100    AS:i:100    XS:i:19
chr1-21 0   chr1    35923261    60  100M    *   0   0   CTAAGCAGCAGTGTTTTTGGATACTTTTTTTTTCTGTTTGTGAATAAGGCCAGCACTCAAGATGGGCAGCCAAGGGTGCACTGACTATTAGCTGGCCCAT    =@@DFDFEHGHHH8JIJGJH1JJHHJIHJGH?IIFEJIIG87JI=IAJJJBJIJD(IIFI8JIHF=JDHEJHEHDDCEDCDEACDDCCAD<BDE+B8(DD    NM:i:0  MD:Z:100    AS:i:100    XS:i:0
chr1-20 16  chr1    112489190   60  100M    *   0   0   AGGGAATGAACTATGCACATCTATATAGTAACAGGGACAGATTTTTTTTTAACATGAGAGTGTAAAAAAAAGAAAAAGAAAAAAAAAGGCCAGGCACAGT    DACDABD@DDDDDA7DDDC8GHI@EI(DC?FG'+8.FBDJIHIEGG=IIG=I@*DFIJJIBIIJIJIIHJCHBGFJJJI@F>HJIIIHHAHFAFDFFC@1 NM:i:1 MD:Z:33A66 AS:i:95 XS:i:28 chr1-19 0 chr1 160371244 60 100M * 0 0 AGGCCCTGGGCACAGGCAGAGAGCCCACCGGCTGGTCATGAGGGCCTCTTCCTTTCTCTGACCCAGGCACCTCGAGGGCTCTTCTCCTGGGTTCCTTCCG @@:FDDFFCHHAHI:GEJFJGF@JJJFIC9JIIJJJ?IIEFHGJ'G?BFFBIIDIG,J)AJIHEGFBHCI&ECCD@EDD?)DED(D>3C?ABEEDDD4BD    NM:i:1  MD:Z:80A19  AS:i:95 XS:i:0
chr1-18 16  chr1    179855835   60  100M    *   0   0   AGCAATTAAAATAAATTAGGGTATCTTTAAAAGTTGTAAAATTATAGCAGTGAAGTACTGTTGACCAGGCACAGTGGCTCACACCTGTAATACCAGCACT    DCEDBBDD/DD9DDD@DDFB(DDDHCHDF;C?;FJGC/IJ8DHEJ:DFGGIGHBIGIJDI(JDHGJJGJIHJII@HJJJ3JIJDIJBBHHFHFDFFF@C@    NM:i:2  MD:Z:20T39C39   AS:i:90 XS:i:34
chr1-17 16  chr1    207455995   60  100M    *   0   0   GGTTCTTATGATTGGAAAGGTTAAAGAGTGACCTATAGGTCACTTTCCAATTATGAAAACAAAAAATTAAGAAATATATATATTTTCATTATTTCACTCC    <DC>CBDDDCDD:&DDCFCFDDHDEJEDCFDJ;;EHGCD;CG?DIHGGCIJJJJ-GIJ7GIFHHHCGI)JJJJIJEGJIGJJJIH<GFGHHBFD@FDCC1    NM:i:0  MD:Z:100    AS:i:100    XS:i:0
chr1-16 0   chr1    114154603   60  100M    *   0   0   TGTATCTTTCTGCTAAGCATAACAAGAAAGACAGAAAGCTCAACGGGAGGATTGAGGCTAGACTTAAAGTAGAGATCCCCTCAGAAACTGTGGAGTGAGG    CCCF8FFDHHHH4JIJIGIJIIFJHJJ?JEDI9BG?I>GHJ7FJJJIF67EIIHD2C>?>DDHDE8E7@JEJ(IFDDC;EDCC:FD>@DBC>D5D>=<AB    NM:i:0  MD:Z:100    AS:i:100    XS:i:0
chr1-15 16  chr1    169767580   60  100M    *   0   0   GGTGGGGGAGAGGAAAGGAAACGAGGGAGGAAAGGCCCTAATAGGGAGGATTTTGGAGTTTAGATTTTAAAATGATAAAGGTTGTTTGACACTCTAGGCA    DEDD9DDD@DD4DDDAEDDDC@D7=D;DA)7;IIJFD(J?JJDGI(IDGD7D'3JIE;H?AC@EHJJE?JJHDFJIIIECG)GGJJECHFHHFDFDFC@C NM:i:2 MD:Z:45A35A18 AS:i:90 XS:i:19 chr1-14 0 chr1 117644126 60 100M * 0 0 GCATTTCATTGTGGACTAATTTTCCCCCACTATTGAGGGAAGACCCTTTTGAGTACTCTATCTGATGCCCCATGAATGATAAAGTTTTATACTCTGGCTG C?CFBDFFGHHH<JAGCIJJIIIGJJI8:JIJC(JGGJBJH-GIJIJJ;IIH;>JI5CJ=CD9DC-HGIJDCJHHDBEDDCC&DDDBD39DBCDDDDDCD NM:i:0 MD:Z:100 AS:i:100 XS:i:0 chr1-13 0 chr1 104996994 60 100M * 0 0 TTCTTGCTGGAACACATGTTTTCACCTTTACCTTCACCCACAGCCCAATGTGCATCAATATGGAGATAATGCAGTTCCATTTATACCTCTTTGTGGTTCA =@?FFFDDHBHHHJHJIJ)JIJ<IJII++>HBHIJ*G:CJCJJJI?G)>GJI;JD3FJ8FJFGD;DDDDFBED7C<?E@C>7&A(ABC9CD+DCC&DDCA NM:i:3 MD:Z:18G63G12A4 AS:i:85 XS:i:22 chr1-12 16 chr1 108617705 60 100M * 0 0 AGGTCGGGGAGATTGGGAAGAAGAATGAGCAAAGAAACCACCAGTGTGATCAGAGGAGGAAAGCAAAGCAGAGTCCTGTCCTGAAAACCAAATGAAGAAA :=>+D(DCEC=@GHB(CDDDDHABDD+HBJJ9F?A35DDIE?JJHIHJJIEE?JFJ?7JBGJJI>JJGJBJIIBIJJJIIIIJGJGJHHDFHF3FFFCB@ NM:i:0 MD:Z:100 AS:i:100 XS:i:0 chr1-11 16 chr1 72085324 60 100M * 0 0 TACTAGCCTTGAAAATGTTTAAAATAATATTCCAGAGTTAATATTGTTGTCCCTGGTATGTTAAAGAGTATTTGTTATCATAGCCAATTCTTGAGTCTGC 8@DDCD4D>D?C3DF(DCCHDDDA;HDEIBFCHGHHHFFIFEG1JHIJIJCGEJIHJG)IH(IJ)BDJ??FHHJHCJJIFJHJJJGIGH)2HFFFA=<CC NM:i:2 MD:Z:61G27A10 AS:i:90 XS:i:0 chr1-10 16 chr1 214311330 60 100M * 0 0 GGTGATCCCTATTTGGTCCACTTTTGTTGGTAGTCTTCAAGCTTGATATCTGATTATCACTGTTGGAAGGTGTAAACTCACAGACTCAGAATTCTGGACT D&++DDEDDC3+CB(@D8DDEHD;?FDF?DBI9A7@JFHJ(I(AIJJ@DHJIIJHII>HFG1JJFII<IJJJ(<HIJGHIJJE=FH@FHHHHFDDDFC@C NM:i:1 MD:Z:1A98 AS:i:98 XS:i:0 chr1-9 0 chr1 152012629 60 100M * 0 0 AAGCAATTCTCTTGCTTTAGCCTCCCGAGAAGCTCGGATTACAGGCATGTCCACCACACCCAGCTAATTCTTTTGTATTTTTAGTAGACATGGGGTTTTG BC:FFDFFHHHFHJ9HIJHIJJJFIGJCI=H/IHH@IGJIJIJIGJJJJEEIB'JJDJJIJDIGICHEFD@D3:0A/(BECDDDDCBE>BD8DDDDDC8C    NM:i:0  MD:Z:100    AS:i:100    XS:i:38
chr1-8  0   chr1    79478960    60  100M    *   0   0   GGCAACACTTGAGAACACAAAGTGAGTTCTCACTTTGGGCGGTGGTTTCAGGCTTCAGGGTGGAGTTTTGTCAGGAACCCAACCTTTTCTGCCTAGAATT    @CCFDFDADHHAHFIH@ICJJHI5?JIJ)GCFEIJHG=II)HIGI9JJIJGHEJHFI8EIDG)GCI4FJF?I8HCDH;DD0&3CFDDDD@C4DCD6ADD>    NM:i:1  MD:Z:81C18  AS:i:95 XS:i:0
chr1-7  0   chr1    178190761   60  100M    *   0   0   GTAGCCGGAATAAACAGTCACTGTGAGTTGTCCATTTTAGAGCATAGGTTTTCAGGTGGTGAAGACCTGTCCTTAGTTGAATTTGTATGTGAATTAAACT    B?<;FDDFHHHHHJEGJFCJJIFJJJA=JHHGIIGJJIIGIGJ(D:DAFG7)&DJID9J)FCD/HHJEDFIJ<FJAF@D@DDADF?C@A@ADCDD@CDDD    NM:i:1  MD:Z:52T47  AS:i:95 XS:i:0
chr1-6  0   chr1    42572411    60  100M    *   0   0   AACCCTTTATCAGGTATGTATTATAAACATCGACTCTGTGGCTTGCATTTTCATTCTCCTTATATATCTTTTGATGAATCAAAGTTTTTAATTTGAATAT    BCCFFFFDAH)HHJJHIGG,HFH2JIJJ4IDI93IJJ<=JJ>IH7IJIJBIBG)CFH7DHHFAHFHEDIFBEFH;EBICA?3DD5D(DDBACDC(BADD:    NM:i:1  MD:Z:94T5   AS:i:95 XS:i:19
chr1-5  0   chr1    153186635   46  100M    *   0   0   GTCTTGACTCTTTATCCACTTTGCCAGTCTGTGTCTTGTAATTGGGGCATTTAGCCTATTTACATTTAAGGTTAATATTGTTATGTGTGAATTTGATCCT    C1@?FF=FHHHHHI?JJEJFIIIHG:.?>EEJEI(JG9J'IIHIJIHIJGJGJFJ9FJAG4EEC:DADE8DAEJFCCBBCDAEDDDD-DDDD@+DBC8D+ NM:i:0 MD:Z:100 AS:i:100 XS:i:82 XA:Z:chr1,-50361546,4; chr1-4 16 chr1 121241807 0 100M * 0 0 AAATGGAGCCTTGAACCAAATAAATAGGTAGTAAAGGTAAATTTAAAATATGAAAAAAAATTTTTTTGAAAGGCATATGTTGACGAGTATTATTTCCACT CCDD<BDHCC:2DE>DDH3BDE(?5ED=GC(GBCJ>JDJDIAJ>DBGDFEDFIFIJJC?80IJ?GIFIIJIIJJJ+JC92JJJJIIJHDHFHDDFFFC:C NM:i:0 MD:Z:100 AS:i:100 XS:i:100 XA:Z:chr1,+145038405,0;chr1,-206260326,74M2D26M,2;chr1,-144029446,52M1I47M,1; chr1-3 0 chr1 84685021 60 100M * 0 0 TATTATTAAAACTATAAATGGACCAATTAAACAAACGTGTCATGAGCCAAGGAATATAAACTAATTCTTTACACCTGAAGTCCTTTAAAATGATTTAATT CCCDFFB=HHHFHJIIJ:E@J>JA2C<IEI2DGJHJGI8FJJIJJAHJCIJJJJ*JJ;F?HFDJCIJFDJ'CHECDEFD,DDFBCH<A7DCD-DDBDD(E    NM:i:2  MD:Z:92C5A1 AS:i:93 XS:i:20
chr1-2  0   chr1    62477842    60  100M    *   0   0   TAGGAAAATGGAGAAACTTTAATATGAAATCTTCCTGTTTTTCACATTATGTTTAGATTGTTACAGCATAAAATTTCAGAAACATTGCAAAAAGTTTTAA    @C=FFDFDHHHH>GJ@IEJGJIIJJJJF@JHHIIGGJ<IHJIG/J*?GD>ICIAJFJIH)H7E?GHEDI>HHFAHC@)(D>DDDED<DCDD=DBBC5DDE    NM:i:2  MD:Z:77C0A21    AS:i:90 XS:i:0
chr1-1  0   chr1    11355150    60  100M    *   0   0   ATTTATTGGCTGTCTTTCAGGCACATTTTAGCTGTCATCCAACATTCTCAACCTTAGTCCCCTTCTCTGGGCTAAGGGGAGAATGATGGTCCTACCCCAG    BC?DFFFFHH<FFJG(ICJIGJJIJJGIFJIJGJ(7FJJJJJFDHID)JCH=3DIJ5JGDI8@@I@A=>3<:IDCA9DDFFI(FADEBDCDCCDDB(DC>    NM:i:0  MD:Z:100    AS:i:100    XS:i:0
hadoop@Master:~/cloud/adam/xubo/data/GRCH38Sub/cs-bwamem$

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